GFAP Antibody / Glial Fibrillary Acidic Protein
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
Product Specifications
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P14136
Host
Mouse
Reactivity
Human
Immunogen
A recombinant full-length human GFAP protein was used as the immunogen for the GFAP antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
GFAP/9424
Applications
IHC-P, WB
Purity
Protein G affinity
Format
Purified
Limitations
This GFAP antibody is available for research use only.
Storage Conditions
Aliquot the GFAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the GFAP antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/9424) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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