Glucose-6-phosphate isomerase Antibody / GPI

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P06744

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human full-length GPI protein was used as the immunogen for the Glucose-6-phosphate isomerase antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

GPI/2439

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This Glucose-6-phosphate isomerase antibody is available for research use only.

Storage Conditions

Aliquot the Glucose-6-phosphate isomerase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Glucose-6-phosphate isomerase antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human hepatocellular carcinoma tissue with Glucose-6-phosphate isomerase antibody (clone GPI/2439) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.