PHPT1 Antibody / Phosphohistidine phosphatase 1

PHPT1 (phosphohistidine phosphatase 1), also known as 14 kDa phosphohistidine phosphatase, is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTAinsensitive phosphohistidine phosphatase. First identified in human adrenal gland, PHPT1 is highly expressed in skeletal muscle and heart, with lower expression in liver, pancreas and kidney. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. PHPT1 is highly conserved among species, suggesting that it serves an essential functional role.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q9NRX4

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human 14 kDa phosphohistidine phosphatase protein was used as the immunogen for the PHPT1 antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

PHPT1/12053

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Limitations

This PHPT1 antibody is available for research use only.

Storage Conditions

Aliquot the PHPT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the PHPT1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human hepatocellular carcinoma tissue with PHPT1 antibody (clone PHPT1/12053) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.