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Hexokinase 1 Antibody / HK1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P19367

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-250 from human HK1 protein was used as the immunogen for the Hexokinase 1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

HK1/9514

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This Hexokinase 1 antibody is available for research use only.

Storage Conditions

Aliquot the Hexokinase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/hexokinase-1-antibody-hk1-hk19514-v5725

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Hexokinase 1 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human brain tissue with Hexokinase 1 antibody (clone HK1/9514) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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