Hexokinase 1 Antibody / HK1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

Product Specifications

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P19367

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-200 from human HK1 protein was used as the immunogen for the Hexokinase 1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

HK1/9510

Applications

WB, IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This Hexokinase 1 antibody is available for research use only.

Storage Conditions

Aliquot the Hexokinase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Hexokinase 1 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human brain tissue with Hexokinase 1 antibody (clone HK1/9510) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.