IGF1R alpha Antibody / Insulin like growth factor 1 receptor alpha

This antibody is specific for the human insulin-like growth factor 1 receptor (IGF1R) . It recognizes the extracellular portion of the receptor, specifically an epitope in the cysteine rich domain of the alpha subunit, between amino acids 184 and 283. It does not cross-react with rat or mouse IGF1R or human insulin receptors in co-precipitation or immuno-depletion assays. The ability of this antibody to bind human IGF1R has been confirmed in IGF1 binding assays using both IG1FR-overexpressing IGF1R/3T3 cells and soluble receptors (Soos et al, 1992) . This antibody efficiently precipitates human IGF1R solubilized from IGF1R/3T3 cells (Soos et al, 1992) and human placental membranes (Bailyes et al, 1997), and has been used in immunoprecipitation analysis of 293 cells transfected with IGF1R or chimeric IGF1R-insulin receptor (IR) constructs (Schumacher et al, 1993) . When immobilized, this antibody has been shown to remove essentially all soluble, IGF1R/3T3-derived IGF1R (Soos et al, 1992) and human placental membrane-derived IGF1R (Soos et al, 1993) in immuno-depletion experiments. This antibody strongly inhibits IGF1 binding to its receptor (Soos et al, 1992), and has been shown to suppress IGF1-mediated stimulation of skin fibroblast proliferation (Okubo et al 2003) .