Galectin 3 Antibody / LGALS3

Galectin-3 is a member of the beta-galactosidase-binding lectin family. It is associated with cell growth, adhesion, inflammation, mRNA processing, and apoptosis. Aberrant expression of Galectin-3 is related to malignant transformation and metastasis in carcinomas of the breast, colon and thyroid. Galectin-3 reactivity can be seen in the nucleus of neutrophils, vascular endothelium, carcinomas of the colon, breast, and thyroid. Galectin-3 may be useful in the differentiation of benign and malignant thyroid neoplasms. Galectin-3 may also be useful in the identification of certain liver disorders.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P17931

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-250 from human LGALS3 protein was used as the immunogen for the Galectin 3 antibody.

Clonality

Monoclonal

Isotype

IgG2b λ

Clone

LGALS3/7145

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This Galectin 3 antibody is available for research use only.

Storage Conditions

Aliquot the Galectin 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Galectin 3 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human colon tissue with Galectin 3 antibody (clone LGALS3/7145) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.