ATM Antibody / Ataxia telangiectasia mutated

The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiec-tases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q13315

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1900-2500 from human ATM protein was used as the immunogen for the ATM antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ATM/9472

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Limitations

This ATM antibody is available for research use only.

Storage Conditions

Aliquot the ATM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ATM antibody should be determined by the researcher.

Location

Cytoplasm, Cytoplasmic vesicle, Nucleus

Image Legend

IHC staining of FFPE human adrenal gland tissue with ATM antibody (clone ATM/9472) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.