NDPK-B Antibody / Nucleoside diphosphate kinase B / NME2

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP) . In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P22392

Host

Mouse

Reactivity

Human

Immunogen

A recombinant full-length human Nucleoside diphosphate kinase B protein was used as the immunogen for the NDPK-B antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NME2/6436

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This NDPK-B antibody is available for research use only.

Storage Conditions

Aliquot the NDPK-B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the NDPK-B antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human kidney tissue with NDPK-B antibody (clone NME2/6436) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.