Keratin 20 Antibody / Cytokeratin 20 / CK20
This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20) . KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
Product Specifications
Specifications
Western blot: 2-4 µg/mL
UniProt
P35900
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein from human KRT20 protein (within amino acids 196-323) was used as the immunogen for the Cytokeratin 20 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
KRT20/3224R
Applications
WB
Purity
Protein A affinity
Format
Purified
Limitations
This Cytokeratin 20 antibody is available for research use only.
Storage Conditions
Aliquot the Cytokeratin 20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Cytokeratin 20 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human HT29 cell lysate with Keratin 20 antibody (clone KRT20/3224R) . Predicted molecular weight ~46 kDa.
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