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Recombinant BAX Antibody

Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Product Specifications

Specifications

Western blot: 2-4 µg/mL

UniProt

Q07812

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-100 from human BAX protein was used as the immunogen for the recombinant BAX antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

BAX/3158R

Applications

WB

Format

Purified

Limitations

This recombinant BAX antibody is available for research use only.

Storage Conditions

Aliquot the recombinant BAX antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant BAX antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot testing of human A549 and MCF7 cell lysate with recombinant BAX antibody (clone BAX/3158R) . Predicted molecular weight ~21 kDa.

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