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Recombinant BCL2L4 Antibody / BAX

Recognizes a protein of 21 kDa, identified as the Bax protein (also called Bcl-2-like protein 4 or BCL2L4) . This MAb is highly specific to BCL2L4 and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. BCL2L4 has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of BCL2L4 accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and BCL2L4 also counters the death repressor activity of Bcl-2.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

Q07812

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-100 from human BAX protein was used as the immunogen for the recombinant BCL2L4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

BAX/9925R

Applications

IHC-P, WB

Format

Purified

Limitations

This recombinant BCL2L4 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant BCL2L4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/recombinant-bcl2l4-antibody-bax-bax9925r-v5784

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant BCL2L4 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human liver tissue with recombinant BCL2L4 antibody (clone BAX/9925R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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