Cyclin D1 Antibody

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P24385

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 100-295 from human Cyclin D1 protein was used as the immunogen for the Cyclin D1 antibody.

Clonality

Monoclonal

Isotype

IgG κ

Clone

CCND1/12039

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Limitations

This Cyclin D1 antibody is available for research use only.

Storage Conditions

Aliquot the Cyclin D1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human thyroid tissue with Cyclin D1 antibody (clone CCND1/12039) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.