S100A4 Antibody / S100 calcium-binding protein A4

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, Î±6Î²4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P26447

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-101 from human S100A4 protein was used as the immunogen for the S100A4 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

S100A4/6802

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This S100A4 antibody is available for research use only.

Storage Conditions

Aliquot the S100A4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the S100A4 antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human spleen tissue with S100A4 antibody (clone S100A4/6802) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.