NKX2.1 Antibody / TFF-1 / Thyroid transcription factor 1

Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1) and Homeobox protein Nkx-2.1. NKX2.1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. NKX2.1 reactivity is also seen in thyroid malignancies.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P43699

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-200 from human TTF-1 protein was used as the immunogen for the NKX2.1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

NX2.1/9033

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This NKX2.1 antibody is available for research use only.

Storage Conditions

Aliquot the NKX2.1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the NKX2.1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human thyroid tissue with NKX2.1 antibody (clone NX2.1/9033) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.