Recombinant TLE1 Antibody / Transducin-like enhancer protein 1

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q04724

Host

Mouse

Reactivity

Human

Immunogen

A recombinant full-length human TLE1 protein was used as the immunogen for the recombinant TLE1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RTLE1/13032

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This recombinant TLE1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant TLE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant TLE1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human endometrium tissue with recombinant TLE1 antibody (clone rTLE1/13032) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.