VEGFA Antibody / Vascular Endothelial Growth Factor A

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF) . It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF) . There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P15692

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-200 from human VEGF protein was used as the immunogen for the VEGFA antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

VEGF/9366

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This VEGFA antibody is available for research use only.

Storage Conditions

Aliquot the VEGFA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the VEGFA antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface and extracellular (secreted)

Image Legend

IHC staining of FFPE human placenta tissue with VEGFA antibody (clone VEGF/9366) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.