Vascular Endothelial Growth Factor A Antibody / VEGFA

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF) . It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF) . There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P15692

Host

Mouse

Reactivity

Human

Immunogen

Human VEGF189 recombinant protein was used as the immunogen for the Vascular Endothelial Growth Factor A antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

VEGF/9368

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This Vascular Endothelial Growth Factor A antibody is available for research use only.

Storage Conditions

Aliquot the Vascular Endothelial Growth Factor A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Vascular Endothelial Growth Factor A antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface and extracellular (secreted)

Image Legend

IHC staining of FFPE human placenta tissue with VEGFA antibody (clone VEGF/9368) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.