CALB1 Antibody / Calbindin

The family of EF-hand type Ca2+-binding proteins includes Calbindin D28K, Calbindin D9K, S-100 (also designated oncomodulin) . Calbindin D28K, also known as calbindin, CALB1, D-28K or vitamin D-dependent calcium-binding protein, is a 261-amino acid protein with 6 EF-hand domains, 4 of which are active calcium-binding domains. Expressed in brain, ovary, uterus, testis, pancreas, liver, kidney and intestine, Calbindin D28K acts as a calcium-buffering agent and alters the activity of the plasma membrane ATPase. In neuronal cells, Calbindin D28K modulates calcium channel activity, calcium transients and intrinsic neuronal firing activity. Also, Calbindin D28K has been implicated to play a role in apoptosis and microtubule function.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P05937

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 7-96 from human Calbindin protein was used as the immunogen for the CALB1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KR6

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This CALB1 antibody is available for research use only.

Storage Conditions

Aliquot the CALB1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CALB1 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human tonsil tissue with CALB1 antibody (clone KR6) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.