Recombinant Calretinin Antibody / CALB2 / Calbindin 2

It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2) . Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.

Product Specifications

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P22676

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human Calretinin partial protein (within amino acids 23-242) was used as the immunogen for the recombinant Calretinin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CALB2/4688R

Applications

WB, IHC-P

Purity

Protein A affinity

Format

Purified

Limitations

This recombinant Calretinin antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Calretinin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Calretinin antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human brain tissue with recombinant Calretinin antibody (clone CALB2/4688R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.