Tissue factor pathway inhibitor 2 Antibody / TFPI2

The extrinsic pathway of blood coagulation is initiated by contact of plasma factor VII with tissue factor, a cellular membrane glycoprotein that normally is segregated from the bloodstream but can be exposed after tissue injury or newly synthesized in endothelial cells or leukocytes after stimulation by endotoxin and cytokines. Inhibition of Factor VIIa tissue factor activity requires a plasma component (tissue factor pathway inhibitor (TFPI), lipoprotein-associated coagulation inhibitor (LACI) or extrinsic pathway inhibitor (EPI) ) and factor Xa. TFPI directly inhibits factor Xa, and, in an Xa-dependent fashion, also inhibits the Factor VIIa tissue factor catalytic complex. TFPI is a multivalent, Kunitz-type proteinase inhibitor that circulates in association with plasma lipoproteins VLDL, LDL, and HDL. TFPI-2 (also known as placental protein 5) is a related glycoprotein that was originally isolated from human placenta.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P48307

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 100-235 from human TFPI2 protein was used as the immunogen for the Tissue factor pathway inhibitor 2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TFPI2/13027

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This Tissue factor pathway inhibitor 2 antibody is available for research use only.

Storage Conditions

Aliquot the Tissue factor pathway inhibitor 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Tissue factor pathway inhibitor 2 antibody should be determined by the researcher.

Location

Cell membrane, cytoplasm

Image Legend

IHC staining of FFPE human placental tissue with Tissue factor pathway inhibitor 2 antibody (clone TFPI2/13027) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.