BRCA1-Associated Protein 1 Antibody / BAP1

The BAP1 gene belongs to the ubiquitin C-terminal hydrolase subfamily of de-ubiquitination enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germ line mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, Uveal melanoma and cutaneous melanoma.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q92560

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 400-729 from human BAP1 protein was used as the immunogen for the BRCA1-Associated Protein 1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

BAP1/13501R

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Limitations

This BRCA1-Associated Protein 1 antibody is available for research use only.

Storage Conditions

Aliquot the BRCA1-Associated Protein 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the BRCA1-Associated Protein 1 antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human breast carcinoma tissue with BAP1 antibody (clone BAP1/13501R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.