CD83 Antibody
CD83 is a heavily glycosylated membrane protein of the immunoglobulin (Ig) superfamily that is expressed in mature Dendritic cells, Langerhans cells and interdigitating reticulum cells within lymphoid tissues. Structurally, CD83 resembles other Ig superfamily members, which have an extracellular V-type Ig-like domain, a single transmembrane domain and a 40 residue cytoplasmic tail. CD83 expression is used as a marker for mature, antigen presenting Dendritic cells that are capable of generating tumor-specific T cell immunity, a phenotype with implications as an anti-cancer vaccine. CD83-IgG1 (Fc) chimera studies indicate that CD83 is a sialic acid-binding, Ig-like Lectin (Siglec) adhesion molecule that is involved in cell adhesion/signaling by hosting Dendritic cell interactions with monocytes and CD8+ T cells.
Product Specifications
Specifications
Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
Q01151
Host
Mouse
Reactivity
Human
Immunogen
Clonality
Monoclonal
Isotype
IgG1 k
Clone
1H4b
Applications
WB, IHC-P
Purity
Protein G affinity
Format
Purified
Limitations
This CD83 antibody is available for research use only.
Storage Conditions
Aliquot the CD83 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the CD83 antibody should be determined by the researcher.
Location
Cytoplasm, Membrane
Image Legend
Western blot testing of human RH30 and PC-3 cell lysate with CD83 antibody. Expected molecular weight: ~23-60 kDa depending on level of glycosylation.
Frequently Asked Questions
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