Recombinant CD19 Antibody

CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.

Product Specifications

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P15391

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of human CD19 protein (around aa1-300) was used as the immunogen for the recombinant CD19 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 κ

Clone

CD19/4260R

Applications

WB, IHC-P

Format

Purified

Limitations

This recombinant CD19 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant CD19 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant CD19 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using recombinant CD19 antibody (clone CD19/4260R) . These results demonstrate the foremost specificity of the CD19/4260R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.