HPV-16 Antibody / E6 protein

Reacts with E6 protein of human papilloma virus type 16 (HPV-16) .</strong> Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

HPV-16 E6 peptide MHQKRTAMFQDPPQERPRKLPQLC was used as the immmunogen for the HPV-16 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

4B12

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This HPV-16 antibody is available for research use only.

Storage Conditions

Aliquot the HPV-16 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the HPV-16 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human cervix tissue with HPV-16 antibody (clone 4B12) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.