Recombinant ANO1 Antibody / DOG1 / TMEM16A

Expression of DOG1/ANO1 protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for ANO1 has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of ANO1 and c-kit in GIST's is nearly identical: 94.4% vs. 94.7%.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q5XXA6

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human full length protein was used as the immunogen for this recombinant ANO1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RDG1/8750

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Limitations

This recombinant ANO1 antibody is available for research use only.

Storage Conditions

Store the recombinant ANO1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell Surface and Cytoplasmic

Image Legend

IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with ANO1 antibody (clone rDG1/8750) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.