Biotinylated Mouse IL-23 alpha & IL-12 beta Heterodimer Protein, His, Avitag™&Tag Free (MALS verified)

Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Product Specifications

Background

Specifications

Biotinylated Mouse IL23A & IL12B Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 23.2 kDa (IL23A) and 35.8 kDa (IL12B) . Subunit IL23A is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL12B contains no tag at the C-terminus. The protein migrates as 27 kDa, 42 kDa and 43-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Val 22 - Ala 196 & Met 23 - Ser 335

Target

IL23A & IL12B

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi | Native

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.