Unconjugated Human HLA-A*01:01&B2M&MAGEA3 (EVDPIGHLY) Complex Protein (Monomer, MALS verified)

The HLA-A*01:01 allele is a prevalent human MHC class I variant, and its peptide-binding specificity determines the immune response in individuals carrying this allele. MAGEA3 is a member of the Melanoma-Associated Antigen family, characterized by its restricted expression in normal tissues (primarily testes and placenta) but frequent re-expression in various tumors, such as melanoma, lung cancer, and others.

Product Specifications

Background

Specifications

Unconjugated Human HLA-A*01:01&B2M&MAGEA3 (EVDPIGHLY) Complex Protein is produced by co-expression of HLA-A*01:01 and B2M loaded with MAGEA3 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated. The protein has a calculated MW of 36.0 kDa and 13.8 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Gly 25-Thr 305 (HLA-A*01:01) & Ile 21 - Met 119 (B2M) & EVDPIGHLY

Target

HLA-A*0101 & B2M & MAGEA3 (EVDPIGHLY)

Conjugation

Unconjugated

Tag

C-10xHis & C-Avi

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.