Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free (MALS verified)

Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Product Specifications

Background

Specifications

Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.5 kDa (IL23A) and 35.9 kDa (IL12B) . Subunit IL-23A is fused with a polyhistidine tag at the C-terminus and subunit IL-12B contains no tag. The protein migrates as 23-25 kDa and 43-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Val 22 - Ala 196 & Met 23 - Ser 335

Target

IL23A & IL12B

Conjugation

Unconjugated

Tag

C-10xHis | Native

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.