Mouse IL-12B&IL-12A Heterodimer Protein, Tag Free&His Tag (MALS verified)

Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40) . IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.

Product Specifications

Background

Specifications

Mouse IL-12B&IL-12A Heterodimer Protein, Tag Free&His Tag, produced by co-expression of IL-12B and IL-12A, has a calculated MW of 35.8 kDa (IL-12B) and 23.6 kDa (IL-12A) . Subunit IL-12B is no tag and subunit IL-12A contains a polyhistidine tag at the C-terminus. The protein migrates as 28-30 kDa, 42-43 kDa and 45-46 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Met 23 - Ser 335 (IL-12B) & Arg 23 - Ala 215 (IL-12A)

Target

IL-12B & IL-12A

Conjugation

Unconjugated

Tag

Native | C-10xHis

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.