Biotinylated Human Complement Factor B Protein, His, Avitag™ (MALS & SPR verified)

Complement factor B, a component of the alternative pathway (AP) of complement activation. Factor B is one of the upstream, proximal components unique to the AP and is essential for the activation of the pathway. It is an integral component that drives the amplification loop. It is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 86.6 kDa. The protein migrates as 100-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Thr 26 - Leu 764

Target

Complement Factor B

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.