Mouse Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, Flag Tag&Tag Free

Integrin alpha V beta 1 (αVβ1) is a widely expressed adhesion receptor formed by the non-covalent association of the αV and β1 (CD29) subunits. Its activity is regulated by a conformational shift from a folded, low-affinity state to an extended, high-affinity state, a process triggered by divalent cations and intracellular signaling. As a canonical RGD-binding integrin, it interacts with ligands including vitronectin, fibronectin, and osteopontin. This interaction is central to its role in mediating cell migration, where its modest binding affinity enables the cyclic attachment and detachment necessary for cell movement. Furthermore, αVβ1 is implicated in cellular differentiation and is co-opted by pathogens, such as foot-and-mouth disease virus, for host cell entry.

Product Specifications

Background

Specifications

Mouse ITGAV&ITGB1 Heterodimer Protein, Flag Tag&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 112.3 kDa (ITGAV) & 83.5 kDa (ITGB1) . Subunit ITGAV is fused with flag tag at the C-terminus and subunit ITGB1 contains no tag. The protein migrates as 160-180 kDa (ITGAV) and 120-140 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Phe 31 - Val 988 (ITGAV) & Gln 21 - Asp 728 (ITGB1)

Target

Integrin alpha V beta 1

Conjugation

Unconjugated

Tag

C-Flag | Native

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.