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Biotinylated E. coli CheY Protein, His, Avitag™ (MALS verified)

CheY is acetylated at lysine residues 92 and 109 by acetyl-CoA synthase (Acs) . [More information is available at EcoGene: EG10150]. Chemotaxis regulator protein CheY is activated through phosphorylation of an aspartate residue by the CheA (L) -his-P protein.

Product Specifications

Background

CheY is acetylated at lysine residues 92 and 109 by acetyl-CoA synthase (Acs) . [More information is available at EcoGene: EG10150]. Chemotaxis regulator protein CheY is activated through phosphorylation of an aspartate residue by the CheA (L) -his-P protein.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 17.7 kDa. The protein migrates as 15-17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Expression Region

Ala 2 - Met 129

Target

CheY

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Purity

90%

Format

Powder

Buffer

PBS, pH7.4

Molecular Weight

17.7 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

25ug*1

Host or Source

E. coli

Species

E. coli

Protein ID

P0AE67

Preservative

Trehalose

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