Biotinylated E. coli CheY Protein, His, Avitag™ (MALS verified)
CheY is acetylated at lysine residues 92 and 109 by acetyl-CoA synthase (Acs) . [More information is available at EcoGene: EG10150]. Chemotaxis regulator protein CheY is activated through phosphorylation of an aspartate residue by the CheA (L) -his-P protein.
Product Specifications
Background
CheY is acetylated at lysine residues 92 and 109 by acetyl-CoA synthase (Acs) . [More information is available at EcoGene: EG10150]. Chemotaxis regulator protein CheY is activated through phosphorylation of an aspartate residue by the CheA (L) -his-P protein.
Specifications
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 17.7 kDa. The protein migrates as 15-17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .
Expression Region
Ala 2 - Met 129
Target
CheY
Conjugation
Biotin-labeled
Tag
C-10xHis & C-Avi
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Purity
90%
Format
Powder
Buffer
PBS, pH7.4
Molecular Weight
17.7 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
25ug*1
Host or Source
E. coli
Species
E. coli
Protein ID
P0AE67
Preservative
Trehalose
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