SARS-CoV-2 Spike Trimer Protein, His Tag (LP.8.1/Omicron) (MALS verified)

SARS-CoV-2 Spike Trimer Protein, His Tag (LP.8.1/Omicron) (SPN-S5228) is expressed from human 293 cells (HEK293) . It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, D796Y, S939F, Q954H, N969K, P1143L, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P, ins16MPLF, S31del, F186L, R190S, R346T, V445R, F456L, Q493E, K1086R, V1104L) ) . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Product Specifications

Background

SARS‑CoV‑2 LP.8.1 is an Omicron sublineage first detected through genomic surveillance, carrying distinctive spike protein mutations linked to enhanced immune escape and potential increases in transmissibility. This variant remains under close monitoring by public health agencies for its impact on vaccine effectiveness, therapeutic antibody response, and spread dynamics, contributing valuable insight to ongoing COVID‑19 research and control strategies worldwide.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.8 kDa. The protein migrates as 140-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Val 16 - Pro 1213

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Format

Powder

Buffer

0.1 M Sodium citrate, pH5.5

Molecular Weight

137.8 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

1mg*1

Host or Source

HEK293

Species

SARS-CoV-2

Protein ID

QHD43416.1 (T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, D796Y, S939F, Q954H, N969K, P1143L, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P, ins16MPLF, S31del, F186L, R190S, R346T, V445R, F456L, Q493E, K1086R, V1104L)