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Biotinylated Human LC3A Protein, His, Avitag™ (HPLC verified)

Microtubule-associated protein 1 light chain 3A (LC3A) is an isoform belonging to the LC3 protein subfamily, encoded by the MAP1LC3A gene. As a crucial component of the cellular autophagy machinery, LC3A is intimately involved in the formation of autophagosomes—double-membraned vesicles that sequester cellular waste for lysosomal degradation. It acts as a structural element and interacts with other autophagy-related proteins to regulate the progression of this essential recycling system, which maintains cellular homeostasis and responds to stress by clearing unnecessary or dysfunctional components.

Product Specifications

Background

Microtubule-associated protein 1 light chain 3A (LC3A) is an isoform belonging to the LC3 protein subfamily, encoded by the MAP1LC3A gene. As a crucial component of the cellular autophagy machinery, LC3A is intimately involved in the formation of autophagosomes—double-membraned vesicles that sequester cellular waste for lysosomal degradation. It acts as a structural element and interacts with other autophagy-related proteins to regulate the progression of this essential recycling system, which maintains cellular homeostasis and responds to stress by clearing unnecessary or dysfunctional components.

Specifications

This protein carries a polyhistidine tag at the N-terminus and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 18.2 kDa. The protein migrates as 20-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Expression Region

Met 1 - Phe 121

Target

LC3A

Conjugation

Biotin-labeled

Tag

N-10xHis & C-Avi

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Purity

90%

Format

Powder

Buffer

PBS, pH7.4

Molecular Weight

18.2 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

E. coli

Species

Human

Protein ID

Q9H492

Preservative

Trehalose

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