Biotinylated Human LC3B Protein, Avitag™, His Tag (MALS verified)
LC3B is a pivotal protein encoded by the MAP1LC3B gene, widely recognized as a core component and indicator of cellular autophagy. It plays an essential role in autophagosome formation and the regulation of the autophagic degradation pathway. Critically, the conversion of cytosolic LC3B-I to lipidated, autophagosome-associated LC3B-II upon autophagy activation serves as a robust and widely used biochemical hallmark for assessing autophagic activity.
Product Specifications
Background
LC3B is a pivotal protein encoded by the MAP1LC3B gene, widely recognized as a core component and indicator of cellular autophagy. It plays an essential role in autophagosome formation and the regulation of the autophagic degradation pathway. Critically, the conversion of cytosolic LC3B-I to lipidated, autophagosome-associated LC3B-II upon autophagy activation serves as a robust and widely used biochemical hallmark for assessing autophagic activity.
Specifications
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 18.4 kDa. The protein migrates as 19-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .
Expression Region
Met 1 - Val 125
Target
LC3B
Conjugation
Biotin-labeled
Tag
N-Avi & N-10xHis
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Purity
90%
Format
Powder
Buffer
20 mM Citric acid, 200 mM NaCl, pH3.5
Molecular Weight
18.4 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
25ug*1
Host or Source
E. coli
Species
Human
Protein ID
Q9GZQ8
Preservative
Trehalose
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