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Biotinylated Human LC3B Protein, Avitag™, His Tag (MALS verified)

LC3B is a pivotal protein encoded by the MAP1LC3B gene, widely recognized as a core component and indicator of cellular autophagy. It plays an essential role in autophagosome formation and the regulation of the autophagic degradation pathway. Critically, the conversion of cytosolic LC3B-I to lipidated, autophagosome-associated LC3B-II upon autophagy activation serves as a robust and widely used biochemical hallmark for assessing autophagic activity.

Product Specifications

Background

LC3B is a pivotal protein encoded by the MAP1LC3B gene, widely recognized as a core component and indicator of cellular autophagy. It plays an essential role in autophagosome formation and the regulation of the autophagic degradation pathway. Critically, the conversion of cytosolic LC3B-I to lipidated, autophagosome-associated LC3B-II upon autophagy activation serves as a robust and widely used biochemical hallmark for assessing autophagic activity.

Specifications

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 18.4 kDa. The protein migrates as 19-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Expression Region

Met 1 - Val 125

Target

LC3B

Conjugation

Biotin-labeled

Tag

N-Avi & N-10xHis

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Purity

90%

Format

Powder

Buffer

20 mM Citric acid, 200 mM NaCl, pH3.5

Molecular Weight

18.4 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

25ug*1

Host or Source

E. coli

Species

Human

Protein ID

Q9GZQ8

Preservative

Trehalose

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