Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Monomer, MALS verified)

Ovalbumin (OVA) can induce both humoral and cellular immune responses based on well-characterized peptide epitopes. The OVA257-264 octapeptide was one of the first OVA epitopes to be characterized, it has an amino acid sequence SIINFEKL, which is recognized by cytotoxic T lymphocytes. ovalbumin (Ova) was presented to T cells if it was released from pinosomes into the cytosol by osmotic lysis of pinosomes. Immunization with the adjuvanted SIINFEKL peptide induces long-lasting CD8+ T cell immunity in mice.

Product Specifications

Background

Specifications

Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide. This protein carries a flag tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 39.3 kDa and 17.6 kDa. The protein migrates as 47-50 kDa and 21 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Gly 22 - Met 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide

Target

H-2Kb & B2M & OVA (SIINFEKL)

Conjugation

Biotin-labeled

Tag

C-Flag & C-Avi

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.