Biotinylated Human HLA-A*02:01&B2M&insB (HLVEALYLV) Complex Protein (Monomer, MALS verified)

Insulin is a heterodimer formed by connecting the A chain (21 amino acids) and B chain (30 amino acids) through two disulfide bonds (A7-B7, A20-B19) . Its three-dimensional structure presents a compact spherical conformation. HLVEALYLV is located at amino acids 10-18 of the B chain, and stabilizes the binding interface between insulin and IR through hydrophobic interactions. By regulating the conformation of insulin, it affects its interaction with downstream signaling molecules. The Human HLA-A*0201 & B2M & insB (HLVEALYLV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and HLVEALYLV peptide of the insB.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*02:01&B2M&insB (HLVEALYLV) Complex Protein is produced by co-expression of HLA-A*0201 and B2M loaded with insB peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.4 kDa and 13.8 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & HLVEALYLV peptide

Target

HLA-A*0201 & B2M & insB (HLVEALYLV)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.