Human Th1/Th2 Cytokine Kit-2 (Flow Cytometry Multiplex Bead Assay)

The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

The Human Th1/Th2 Cytokine Kit-2 is a multiplex bead-based indirect immunofluo-rescence assay, utilizing flow cytometry for data acquisition and classification. The beads conjugated with specific antibody, target protein (IL-2/4/5/10, TNF-α, IFN-γ) in specimens and PE-conjugated detection antibody form a sandwiches complex as bead-analyte-detection antibody. The intensity of PE fluorescence signal in proportion to the target protein in specimens, are assessed by flow cytometry. The present assay has been optimized for quantitatively analysis for the IL-2/4/5/10, TNF-α, IFN-γ in cell culture supernatant, plasma, and serum samples.