Human OX40 / OX40 Ligand Inhibitor Screening Kit (TR-FRET)

1. Unopened kit should be stored at 2°C-8°C upon receiving. 2. Find the expiration date on the outside packaging and do not use reagents past their expiration date. 3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This Human OX40 / OX40 Ligand Inhibitor Screening Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) . Use Europium-chelate labeled OX40 Ligand as the donor and a mixture of biotinylated OX40 and FA labeled streptavidin as the acceptor. — In the absence of inhibitors for human OX40 binding to human OX40 Ligand, the donor and acceptor are in close proximity due to the binding of human OX40 and human OX40 Ligand. Upon excitation with a specific light source, the donor emits a 620 nm signal, which is absorbed by the acceptor, resulting in a 665 nm emission. — In the presence of inhibitors that block the binding of OX40 to human OX40 Ligand, the donor-acceptor interaction is disrupted, preventing FRET from occurring.