Human Tumor Necrosis Factor Alpha (TNF-α) Detection Kit (TR-FRET)

Human Tumor Necrosis Factor Alpha (TNF-α) Detection Kit (TR-FRET) is expressed from Human

Product Specifications

Target

TNF-alpha

Applications

The Human Tumor Necrosis Factor Alpha (TNF-α) Detection kit (TR-FRET) can be used to detected the level of human TNF-α protein in the supernatant. It is for research use only.

Stability

1. Unopened kit should be stored at 2°C-8°C upon receiving. 2. Find the expiration date on the outside packaging and do not use reagents past their expiration date. 3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Format

Solid

Components

This human Tumor Necrosis Factor Alpha (TNF-α) detection kit is based on a TR-FRET sandwich assay using two different specific antibodies. One antibody is labeled with a europium chelate (as the donor), while the second is labeled with FA (as the acceptor) . In the presence of human TNF-α, the donor and acceptor are in close proximity because both labeled antibodies bind to the human TNF-α. When excited by a specific light source (337 nm), the donor emits a 620 nm signal, which is absorbed by the acceptor and results in a 665 nm emission.