PE-Labeled Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein

Ovalbumin (OVA) can induce both humoral and cellular immune responses based on well-characterized peptide epitopes. The OVA257-264 octapeptide was one of the first OVA epitopes to be characterized, it has an amino acid sequence SIINFEKL, which is recognized by cytotoxic T lymphocytes. ovalbumin (Ova) was presented to T cells if it was released from pinosomes into the cytosol by osmotic lysis of pinosomes. Immunization with the adjuvanted SIINFEKL peptide induces long-lasting CD8+ T cell immunity in mice.

Product Specifications

Background

Specifications

PE-Labeled Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein is assembled by biotinylated monomer (H2A-M82E6) and PE-labeled streptavidin. Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide. Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) .

Expression Region

Gly 22 - Thr 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide

Target

H-2Kb & B2M & OVA (SIINFEKL)

Conjugation

PE Labeled

Tag

C-10xHis

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.