Anti-Vimentin Antibody

Our Anti-Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Vimentin and is Protein G purified. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006) . Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons) . Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008) .

Synonyms

CTRCT30 antibody, Epididymis luminal protein 113 antibody, FLJ36605 antibody, HEL113 antibody, VIM antibody, VIME_HUMAN antibody, Vimentin antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

VIM

NCBI Gene ID

7431

UniProt

P08670

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Recombinant human vimentin purified from E. coli.

Immunogen Type

Human

Target

Vimentin

Clonality

Monoclonal

Clone

2D1

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

1 mg/ml

Format

Protein G Purified

Form

Liquid

Buffer

PBS + 10 mM NaN3.

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

IHC Dilution

1:500-1:1000

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~50 kDa vimentin protein.

Formulation

Protein G purified culture supernatant.

ICC Dilution

1:500-1:2000

Quality Control

Western blots performed on each lot.

Species

Bovine, Canine, Chicken, Drosophila, Feline, Finch, Fish, Goat, Guinea Pig, Hamster, Horse, Lizard, Non-Human Primate, Rabbit, Sheep, Vole, Xenopus, Zebrafish