Anti-Vesicular GABA transporter (VGAT) Antibody

Our Anti-Vesicular GABA transporter (VGAT) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, mouse, rat, and Xenopus Vesicular GABA transporter (VGAT) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Specifications

Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997) . The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998) . Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005) .

Synonyms

BA122O1.1 antibody, GABA and glycine transporter antibody, hVIAAT antibody, SLC32A1 antibody, SLC32A 1 antibody, Slc32a1 antibody, solute carrier family 32 (GABA vesicular transporter) member 1 antibody, Solute carrier family 32 member 1 antibody, Vesicular GABA Amino Acid Transporter antibody, Vesicular GABA transporter antibody, Vesicular inhibitory amino acid transporter antibody, VGAT antibody, VIAAT antibody, VIAAT_HUMAN antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

SLC32A1

NCBI Gene ID

83612

UniProt

O35458

Host

Rabbit

Reactivity

Chicken, Mouse, Rat, and Xenopus

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat VGAT, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

Vesicular GABA transporter (VGAT)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC Dilution

1:100-1:1000

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~53 kDa VGAT protein.

Formulation

Prepared from pooled rabbit serum by affinity purification on a column made with the peptide used as antigen.

ICC Dilution

1:500

Quality Control

Western blots performed on each lot.

Species

Bovine, Canine, Non-Human Primate

Isotype

IgG

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