Anti-Tryptophan Hydroxylase (Ser260) Antibody

Our Anti-Tryptophan Hydroxylase (Ser260) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Tryptophan Hydroxylase (Ser260) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001) . In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) . CaM K II phosphorylates Ser-260 which lies within the regulatory domain of TPH (Jiang et al., 2000) .

Synonyms

Indoleacetic acid 5 hydroxylase antibody, L tryptophan hydroxylase antibody, MGC119994 antibody, TPH 1 antibody, TPH antibody, TPH1 antibody, TPH1_HUMAN antibody, TPRH antibody, TRPH antibody, Tryptophan 5 hydroxylase 1 antibody, Tryptophan 5 monooxygenase 1 antibody, Tryptophan 5 monooxygenase antibody, Tryptophan 5-hydroxylase 1 antibody, Tryptophan 5-monooxygenase 1 antibody, Tryptophan hydroxylase 1 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

TPH1

NCBI Gene ID

24848

UniProt

P09810

Modification Site

Ser260

Host

Rabbit

Reactivity

Human and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of rat tryptophan hydroxylase (TPH), conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

Tryptophan Hydroxylase (Ser260)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser260. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.