Anti-Tau (Ser416) Antibody

Our Anti-Tau (Ser416) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Tau (Ser416) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Specifications

Background

Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985) . Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990) . Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995) . Ser-416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990) .

Synonyms

AI413597 antibody, AW045860 antibody, DDPAC antibody, FLJ31424 antibody, FTDP 17 antibody, G protein beta1/gamma2 subunit interacting factor 1 antibody, MAPT antibody, MAPTL antibody, MGC134287 antibody, MGC138549 antibody, MGC156663 antibody, Microtubule associated protein tau antibody, Microtubule associated protein tau isoform 4 antibody, Microtubule-associated protein tau antibody, MSTD antibody, Mtapt antibody, MTBT1 antibody, MTBT2 antibody, Neurofibrillary tangle protein antibody, Paired helical filament tau antibody, Paired helical filament-tau antibody, PHF tau antibody, PHF-tau antibody, PPND antibody, PPP1R103 antibody, Protein phosphatase 1 regulatory subunit 103 antibody, pTau antibody, RNPTAU antibody, TAU antibody, TAU_HUMAN antibody, Tauopathy and respiratory failure included antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

MAPT

NCBI Gene ID

69329

UniProt

P19332

Modification Site

Ser416

Host

Rabbit

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser416 of rat tau, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

Tau (Ser416)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

59/65/68

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC Dilution

1:200

WB Dilution

1:1000

Specificity

Specific for ~59, 65, 68 kDa tau protein phosphorylated at Ser416. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.