Anti-Synaptojanin 1 Antibody

Our Anti-Synaptojanin 1 primary antibody from PhosphoSolutions is mouse monoclonal. It detects rat Synaptojanin 1 and is Protein G purified. It is great for use in WB, IHC.

Product Specifications

Background

Synaptojanin is a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms; one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996) . The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down’s syndrome (DS) . It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5- bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008) .

Synonyms

4 antibody, 5-trisphosphate 5-phosphatase 1 antibody, Inositol 5' phosphatase (synaptojanin 1) antibody, Inositol 5' phosphatase antibody, INPP 5G antibody, INPP5G antibody, KIAA0910 antibody, PARK20 antibody, Polyphosphoinositide phosphatase antibody, Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 1 antibody, Synaptic inositol 145 trisphosphate 5 phosphatase 1 antibody, Synaptic inositol-1 antibody, Synaptojanin antibody, Synaptojanin 1 antibody, Synaptojanin 1 polyphosphoinositide phosphatase antibody, Synaptojanin-1 antibody, Synaptojanin1 antibody, Synj 1 antibody, Synj1 antibody, SYNJ1_HUMAN antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

SYNJ1

NCBI Gene ID

85238

UniProt

Q62910

Host

Mouse

Reactivity

Rat

Immunogen

Recombinant protein from the C-terminal region of rat synaptojanin 1.

Immunogen Type

Rat

Target

Synaptojanin 1

Clonality

Monoclonal

Clone

5H1

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Concentration

1 mg/ml

Format

Protein G Purified

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Molecular Weight

145

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC Dilution

1:100

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~145 kDa synaptojanin I protein in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.