Anti-Synapsin I (Ser9) Antibody

Our Anti-Synapsin I (Ser9) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse, rat, and zebrafish Synapsin I (Ser9) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Specifications

Background

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996) . This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994) . Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002) . Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I (Czernik et al., 1987) . Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth (Kao et al., 2002) .

Synonyms

Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

SYN1

NCBI Gene ID

281510

UniProt

P17599

Modification Site

Ser9

Host

Rabbit

Reactivity

Mouse, Rat, and Zebrafish

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

Synapsin I (Ser9)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet protein phosphorylated at Ser9. Also weakly labels the ~55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is completely eliminated by treatment with λ-phosphatase.