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Anti-SAP49 Antibody

Our Anti-SAP49 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat SAP49 and is Protein G purified. It is great for use in WB, IHC, ICC.

Product Specifications

Background

The spliceosome associated protein of ~49 kDa (SAP49), also known as SF3B4, is a widely expressed nuclear splicing factor that has been shown to have RNA binding activity as well as direct interaction with SAP145 (Champion-Arnaud and Reed 1994) . The SAP145-SAP49 complex has recently been implicated in cell cycle progression as Vpr (viral protein 1 of HIV-1) has been shown to bind SAP145 thus interfering with the proper formation and functioning of the SAP145-SAP49 complex (Terada and Yasuda 2006) .

Synonyms

AFD1 antibody, Hsh49 antibody, MGC10828 antibody, Pre mRNA splicing factor SF3b 49 kDa subunit antibody, Pre-mRNA-splicing factor SF3b 49 kDa subunit antibody, SAP 49 antibody, SAP49 antibody, Sf3b4 antibody, SF3B4 antibody, SF3B4_HUMAN antibody, SF3b49 antibody, SF3b50 antibody, Spliceosomal protein antibody, Spliceosome associated protein (U2 snRNP) antibody, Spliceosome associated protein 49 antibody, Spliceosome-associated protein 49 antibody, Splicing factor 3b subunit 4 49kDa antibody, Splicing factor 3B subunit 4 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

SF3B4

NCBI Gene ID

10262

UniProt

Q15427

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Recombinant full length human SAP49 expressed in and purified from E. Coli.

Immunogen Type

Human

Target

SAP49

Clonality

Monoclonal

Clone

3A1

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

1 mg/ml

Format

Protein G Purified

Form

Liquid

Buffer

PBS + 50% glycerol and 5 mm NaN3

Molecular Weight

49

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC Dilution

1:1000

WB Dilution

1:2000

Specificity

Specific for endogenous levels of the ~49 kDa SAP49 protein.

Formulation

Protein G purified culture supernatant.

ICC Dilution

1:1000

Quality Control

Western blots performed on each lot.

Isotype

IgG2b

Frequently Asked Questions

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